AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |
Back to Blog
Tr85.031 firmware download4/4/2023 ![]() The ‘conventional’ wet transfer technique employs the use of a cooling system to reduce heat produced by the electrodes, although over the years modifications have been developed. A western blot of collagen-induced platelet activation over time using semi-dry protein transferWet transfer involves immersing a gel-membrane sandwich in an upright tank of transfer buffer, usually with vertical platinum electrodes. A number of membranes have been developed in the past, but only nitrocellulose and polyvinylidene fluoride (PVDF) remain popular. ![]() Effective protein transfer is also heavily reliant on the gel acrylamide percentage, the molecular weight of electrophoresed proteins, and the blotting membrane used. Electroelution (wet, semi-dry, and dry) and diffusion transfer techniques all have their merits and applications, but it is important to note that method alone will not guarantee a successful blot. The protein transfer stage should carry equal risks to PAGE, although many labs adopt the method depicted in of the aforementioned article, which introduces further possibility of electric shock.There are many safer methods for transferring proteins to a membrane. ![]() The buffer should be covered at all times when attached to the power supply to prevent contact with the electrified liquid. ![]()
0 Comments
Read More
Leave a Reply. |